How Does the Moisture Level of Milkweed Affect the Growth of Caterpillars?
McGuire Jr. High School
Lakeville, MN
Abstract
The purpose was to see how the caterpillars grow in response to different moisture
levels of milkweed. I put the leaves in a box with two sides: dry leaves in one
side, leaves with 2 spritz of water on the other side. We placed caterpillars in
each side. Then we recorded length of caterpillars in cm and recorded observations
everyday. We placed the box in back of the science room by the window. We checked
the box daily for changes in the caterpillars.
The caterpillars with 2 spritz of water and the caterpillars with no spritz of water
grew the same length, in general. Problem: the caterpillars went into pupae unexpectedly.
Also, being close to the window, the sun may have dried out the leaves. The test
ran smoothly, and we found out that the moisture level of milkweed doesn't affect
the caterpillars' growth.
Hypothesis
We believe that the group of caterpillars with two spritz of water will grow the
largest because caterpillars eat moist leaves in the wild. We don't want the leaves
to get too wet for they would grow mold and if they were too dry the leaves would
die. In conclusion the caterpillars will grow larger with two spritz of water, because
they eat moist leaves in the wild and and two spritz isn't too wet or too dry compared
to what they eat in their natural habitat.
Background Information
There are five types of milkweed in the Northern United States: Showy, Swamp, Common,
Whorled, and Butterfly milkweed. It grows from 60-200 centimeters in places like
ditches, fields and swamps. Milkweed makes the caterpillars grow 2,700 times larger
than when they first emerge from the egg. After the caterpillars have eaten the
milkweed, it will grow back in a few weeks so the caterpillars can always go back
to a certain spot to get food. If milkweed is too dry it will die. If it is too
wet it will grow mold. Two spritz of water is the perfect moisture level for the
caterpillars since that is about how much moisture there would be in the wild. Milkweed
is moist in the wild because of rain and morning dew. Since humans need about eight
cups of water each day, we think that caterpillars might need water also.
Materials
- one box split into two sections
- six milkweed leaves each day
- two caterpillars
- spray bottle
- paper towels
- ruler
- data chart
Constants
- number of leaves
- number of spritzes to each leaf
- all larvae start around same size or instar
- moist towels in each side (A & B)
Dependent Variable
The growth of each caterpillar
Independent Variable
The moisture level of the leaves
Procedure
- Get box with two equal sides
- Put wet paper towel in both sides
- Put dry leaves on one side
- Put leaves with 2 spritz of water on the other side
- Put one 1.3 cm caterpillar in each side
- Record length of caterpillars with ruler in cm
- Record observations of caterpillars by size and body features
- Put box back in the back corner by the window
- Feed caterpillars everyday
- Clean and check box daily
Results/Conclusions
Our hypothesis ended up being wrong. The caterpillars with zero spritz, and the
caterpillars with two spritz of water on their leaves actually grew about the same
size. This information can be seen on the graphs.


There were some unusual occurrences. Our first set of caterpillars pupated earlier
than expected, forcing the experiment to be tried again. In the second experiment,
we got results though the caterpillars pupated around the sixth day of observations.
If we did this experiment again, we would add one more independent variable, the
amount of light the caterpillars receive. We believe this would make the conditions
even more like their natural habitat for the caterpillars.
The experiment went very well. We learned that although caterpillars are very different
than us, they still need some of the same things as humans. The experiment was done
to see if the moisture level of the leaves would effect the caterpillars' growth.
Things ran smoothly and we found that the moisture level of milkweed does not affect
the caterpillars' growth.